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Purpurin from Rubia tinctorum also demonstrates comparable antifungal activity (Messier and Grenier, 2011

For the expression of cell death inducing factors, Agrobacterium expressing AtMKK5 DD or INF1 was infiltrated with OD 600 = 0.2, and Agrobacterium expressing SsNE2 was infiltrated with OD 600 = 0.6

Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h

Diallyl Trisulfide protects motor neurons from the neurotoxic protein Tdp-43 via activating lysosomal degradation and the antioxidant response

Faseb j 2009

Compounds were formulated with 10% DMSO and 10% Tween-80, and vortexed to obtain a uniform mixture