While its mechanisms are complex and wide-ranging, questions of delivery and dosing remain front of mind for both researchers and clinicians
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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS
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WARNINGS: Patients with early Leber's disease (hereditary optic nerve atrophy) who were treated with cyanocobalamin suffered severe and swift optic atrophy
Comprehensive human studies are required to clarify safety considerations, effectiveness, and potential relevance within clinical and athletic settings (19)