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The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added

This is more physiologically appropriate and likely explains why the combination of PTD-DBM (upstream release) with valproic acid (downstream activation) produces synergistic rather than merely additive effects

The mixture was injected intraperitoneally