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Methods Cell lines and cell culture The human proximal tubular cell line CD10-135 (referred to as CD10 in the manuscript) [14], originally provided by Prof

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Additionally, we have identified varying degrees of interference in experimental studies on ammonia metabolic reprogramming

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Xue, P., Nga, D

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Miao CY, Fang XJ, Chen Y, Zhang Q

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However, practical protocol design becomes significantly more complex with multi-compound stacks: you must isolate each compounds contribution, monitor for cumulative metabolic stress, and ensure endpoint measurements can differentiate between GH-mediated effects (lipolysis, lean mass changes) and AMPK-mediated effects (insulin sensitivity, fat oxidation)

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