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Interestingly, Pseudomonas nitroreducens GGT (PnGGT) exhibits higher hydrolytic activity than transpeptidase activity despite high sequence similarity with EcGGT ( Picrophilus torridus GGT (PtGGT), the replacement of an aromatic residue tyrosine at position 327 by asparagine (corresponding residue in EcGGT) introduced significant transpeptidase activity in PtGGT, while the native enzyme exhibited only hydrolytic activity (Rajput et al., 2013)