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Recently, our group has demonstrated that PD mimetics including 6-hydroxydopamine (6-OHDA), N-methyl-4-phenylpridine (MPP + ) and rotenone suppressed mTOR signalling, accelerated cleavage of caspase-3 and PARP in PC12 cells and primary neurons and subsequently triggered neuronal apoptosis

[DOI] [PMC free article] [PubMed] [Google Scholar] 9.Shabalala S.C., Johnson R., Basson A.K., Ziqubu K., Hlengwa N., Mthembu S.X.H., Mabhida S.E., Mazibuko-Mbeje S.E., Hanser S., Cirilli I., et al

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Following washes with phosphate-buffered saline with Tween (PBST), sections were incubated with appropriate secondary antibodies for 2 h at room temperature