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Purified complex (3 L, ~4 mg/mL) was applied to the grids that were subsequently vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) under 100% humidity at 4 C

doi: 10.1096/fj.02-0752rev 40 Chiorcea-PaquimAM

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Formulated with 0.9% benzyl alcohol, this solution helps inhibit microbial growth, allowing for multi-dose use under proper laboratory handling conditions

However, these clinical trials were conducted in Croatia and published in the early 2000s