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179,180 This was hypothesized to be related to mRNA folding leading to inaccessibility of the translation start site, 179,180 and interestingly this issue was partially or completely alleviated by performing the IVT step in the presence of a ribosomal extract

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[DOI] [PMC free article] [PubMed] [Google Scholar] 86.Yang L.X., Yao Y.Y., Yang J.R., Cheng H.L., Zhu X.J., Zhang Z.J