Nave CD4 + T cells (0.4 10 6 ) were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, CA, USA), 2 mM glutamine, 1% penicillin streptomycin (Gibco, CA, USA), 10 mmol/L N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES, Gibco, CA, USA), and 55 mmol/L 2-mercaptoethanol (Gibco, CA, USA) in 24-well plates
Smokers were further categorized into three smoking intensity subgroups: 15, 610, and 11 cigarettes per day
The common differentially expressed proteins were immunoglobulin heavy constant gamma 2C (Ighg2c) and cysteine-rich protein 2 (Crip2) between EC1 and CS, and NGG1 interacting factor 3-like 1 (Nif3l1), NHL repeat-containing protein 2 (Nhlrc2), and Crip2 between EC2 and CS
cultivated tobacco) Origin and Habitat These herbaceous plants are indigenous to the Americas, Australia, southwest Africa and the South Pacific
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