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Plug-in coolers for cars, also known as electric coolers, are portable refrigerators that can be plugged into a cars 12-volt cigarette lighter socket to keep food and drinks cool while on the go

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Briefly, 5 g of chopped tobacco leaves were inserted into a mortar, and 20 mL of separation medium (0.35 M sucrose, containing 0.05 M Tris-HCl, 5 mM MgCl 2 , 5 mM L-cysteine, 2 mM EDTA Na, pH 7.5) were added

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