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The intracellular antibody cocktail including Perforin-Pe-Cy7 (BioLegend) and Granzyme B-FITC (BioLegend) was added to the cells and incubated for 30 min on ice

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Expanding macrophages and neutrophils demonstrated impaired phagocytic functions to the efficient clearance of bacteria or pathogen, as evidenced by reduced bacterial-stimulated production of superoxide and surface receptor expression, (e.g

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McMenamin, PhD, MPH