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First, to identify the clusters, the deconvolved (5 iterations) and bleached corrected images were filtered using a combination of Laplacian filter (3 pixels), to enhance their contrast, and the Gaussian filter (1 pixel), to reduce the background

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For hypothalamic neuron differentiation, cells were plated on matrigel coated plates in N2B27 medium (Neurobasal A:DMEM/F12 (1:1) (Life Technologies), 1x B27 without Vit

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Donor samples were obtained from the interregional organ allocation reference center repository [Nord Italian Transplant Program (NITp)]

A first-in-human, randomized, double-blind study of safety and pharmacokinetics in adults suggested that NYX-2925 was safe and well tolerated