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The sections were then incubated with primary antibodies against occludin (Abcam, dilution 1:100) and ZO-1 (ThermoFisher, dilution 1:100), diluted in PBS containing 0.2% BSA and 0.1% saponin at 4 C overnight, washed, and incubated for 1 h with Alexa Fluor 647 donkey anti-rabbit IgG secondary antibody (Invitrogen, dilution 1:400)

Tissue Repair and Healing Wound Healing One of the most widely studied potential uses of BPC-157 is in accelerating wound healing

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High level of the c-Met receptor was confirmed in human melanoma cells M14, M4Beu, A375 and Rel3, and the IC 50 for c-Met inhibitor SU11274 was 45 M [69].Contrary to the expected effect of HGF/c-Met inhibitor, in our experiments with the hypermetastatic and highly tumorigenic variant of human melanoma cell line A375 designated as Rel3 we have shown that SU11274 enriched for the melanoma-initiating cells in vivo

[DOI] [PMC free article] [PubMed] [Google Scholar] [37].Wang B, Chen S, Zheng Q