In brief, the PCR reaction mix contained 7.5 l KAPA SYBR FAST qPCR Master Mix (2X), 0.75 l of 5 M forward and reverse primers each, 3.5 l PCR-grade water and 2.5 l of 20-fold diluted cDNA in 15 l total reaction volume
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In particular, GSH catalytically detoxifies from hydroperoxides, peroxynitrite, and lipid peroxides and directly scavenges various oxidant molecules, like superoxide anion, hydroxyl radical, nitric oxide, and carbon radicals
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A total of ten isolates were used to evaluate the ability of MB to inhibit the maturation from ring to schizont
GC-MS was used to measure 2-AB yield