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The inoculum was prepared from lyophilized tissue homogenized in phosphate-buffered saline (PBS) (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 2 mM KH 2 PO 4 , with pH adjusted to 7.4 with HCl) at 1:5 w/v (1 g of leaf to 5 ml of PBS)

these pouches pack a punch with an EXTREMELY STRONG nicotine content of 32.25 mg per pouch, which translates to a staggering 43 mg of nicotine per gram

The body is generous, with six pairs of yellow spots

With a filter that crumbles in your hand and an overpowering chemical tang, these darts are not designed for the novice smoker

37%) but not for respiratory effects (70% vs

Steinberg, editorial board member of HemOnc Today, said in an interview