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The coverslips were subsequently incubated with anti-Flag-M2 (Sigma, F3165, 1:400) and anti-citrate synthase (Cell Signaling, 14309S, 1:200) for 16h at 4 C before washing 3 times with PBS

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doi: 10.3233/JAD-170758

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In mice, aneuploidy increased from approximately 15% to 43% with age, but SIRT2 overexpression lowered spindle defects and misalignment and increased pregnancy rates from 25% to 75% in transgenic females