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studied the mechanism of thrombogenicity in cirrhosis patients, evaluating the relationship between PS on blood cells and endothelial cells and the hypercoagulable state

Ghaemi, A

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Samples were boiled for 5 min at 95 C with loading buffer (10% 2-mercaptoethanol, 4%SDS, 20%glycerol, 0.125M Tris HCl, and 0.004% bromophenol blue PH6.8)

They address completely different biological systems

Cell were then treated with Cy5.5-labeled NP4 for 1 h, 4 h, or 7 h, respectively, washed with PBS, and analyzed by flow cytometry (Beckman Coulter, U.S.A.)