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Suppressed fibroblast function, decreased collagen synthesis and increased MMPs lead to a reduction in extracellular matrix Subsequent studies focusing on estrogens mechanism of action have largely employed ovariectomised (OVX) rodents

For estimation of mitochondrial mass, the fly brains were stained with 20 M N-Nonyl Acridine Orange for 15 min and fluorescence intensity was determined as above

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Maggie Fox, NBC News, December 10, 2018