The analogues were synthesised via Fmoc-solid-phase peptide synthesis, and they were characterised using reverse-phase high-performance liquid chromatography (RP-HPLC), electrospray ionisation mass spectrometry (ESI-MS), Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) spectroscopy
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Chakrapani H, Kalathur RC, Maciag AE, Citro ML, Ji X, Keefer LK, et al
2 and 3)
Duration of nuclear NF-kappaB action regulated by reversible acetylation
After 24 h, cells were lysed and subjected to luciferase activity using a Luc-Pair Duo-Luciferase HS Assay Kit (GeneCopoeia, MD, USA) according to the manufacturers instructions