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Maternal Chitosan Oligosaccharide Supplementation During Late Gestation and Lactation Affects Offspring Growth

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The immobilized Hh proteins were detected with a primary polyclonal anti-Hh antiserum (rabbit IgG, Santa Cruz Biotechnology, United States) and visualized with a secondary peroxidase-conjugated donkey anti-rabbit IgG (Dianova, Hamburg, Germany) followed by chemiluminescent detection

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As expected, the African human SFB 16S rRNA gene sequences fell within the SFB clade when using as an outgroup four Clostridium species representing different Clostridium clades (Fig