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However, the major concern with BPC 157 is the lack of substantial evidence confirming its safety in humans

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Immunohistochemical mapping of nitric oxide synthase in the rat hypothalamus and colocalization with neuropeptides

10.1212/WNL.55.10.1460 Neurology 165 MintzerJ.LancttK

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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue