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Western blot analysis further confirmed that H2O2 treatment led to increased levels of senescence-associated proteins p53, Ser15-phospho-p53, and p21

Available biomarkers of oxidative stress have been summarized in Table 3

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The results indicated that the overall prevalence of zinc deficiency in this cohort was 23.2%, with 72.4% of participants exhibiting dietary zinc intake levels below the estimated average requirement (EAR)

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