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In the presence of another molecule of hydrogen peroxide, Compound II is transformed into the inactive intermediate oxyferrous Compound III, [(Fe II -O 2 (porphyrin)], according to the reaction (Glorieux and Calderon 2017

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Total thiols 5-Chloromethylfluorescein diacetate (5-CMFDA) [482]

Importantly, the retention time and integral of the peak corresponding to Ru(II)-OH remained unchanged, suggestive that this is a catalytic process (Supplementary Fig