Oxidative stress in an assisted reproductive techniques setting
Following rotisserie hybridization and incubation for the appropriately assigned time, 500 ul of each sample was added to 500 ul of ice-cold acetonitrile
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There were indications of enhanced mitochondrial membrane potential and increased mtDNA copy numbers, suggesting that Epithalon might support mitochondrial integrity and functionality as oocytes age
Subsequently, cells were trypsinized, resuspended in 300 l of Hanks balanced salt solution (HBSS, Gibco), strained through a 40 m cell strainer (Falcon tube with cell strainer CAP), and then analysed using a flow cytometer (CytoFLEX and CytExpert 2.4, Beckman Coulter) with a 488-nm laser paired with a 530/30 nm bandpass filter