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Further, the primary antibodies were incubated at 4 C overnight, and the secondary antibodies were incubated at 37 C for 1 h

Two analysts were blinded about treatment strategies and the scores were averaged for statistics

Ulcerative colitis (UC), one of the dominant forms of inflammatory bowel disease, is associated with an increased risk of developing CRC (9)

As animals were obtained in six separate batches due to operational limitations and grouped in cages, the survival curves were plotted using the KaplanMeier method