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20 If attempts to shift expression from inclusion bodies to the soluble fraction fail, insoluble GST fusion proteins sometimes can be purified in the presence of denaturants such as urea, followed by refolding

For the invasion assay, the membrane of the transwell unit was coated with 35 l Matrigel at 37C for 4 h to form a reconstructed basement membrane

Malnutrition affects glucose metabolism, bone strength, and tissue formation, while birth weight and hormonal balance are compromised

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Plasma, liver, and epididymal fat pad samples were harvested for analysis

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