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This system uses selective digestion of sample DNA with methylation-sensitive and methylation-dependent restriction enzymes, followed by quantification of the remaining DNA using real-time PCR

BioMed Research International (2015) 11

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Inflammation grading was carried out independently by 3 researchers according to established protocols (77) and to the following scale: 0= No inflammation, 1= small pockets of cellular infiltrations, 2= small pockets of infiltrations less than 3 cells deep surrounding more than one airway or blood vessel, 3= infiltrations more than 3 cells deep found surrounding more than one but less than 50% of the total airways, 4=most airways surrounded by significant immune cell infiltrations, 5= the majority of airways are surrounded by significant immune cell infiltrations which also are seen present in alveolar beds

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TNF, a key inflammatory cytokine, has been linked to the clinical prognosis of AP (216)