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NNMTs role is to transfer a methyl group to nicotinamide (vitamin B3), creating 1-methylnicotinamide a reaction that consumes cellular methyl donors and depletes nicotinamide adenine dinucleotide (NAD) stores

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For ankle injuries specifically, subcutaneous injection near the affected area at 0.25 to 0.50 mg daily for 4 to 8 weeks represents the most common protocol among researchers and biohackers

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However, arthritis, back pain, and muscle pain may also occur after a vitamin B12 injection

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