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To verify our hypothesis, we applied a combination therapy strategy using one organic drug quercetin and two bioactive metal ions such as Zn 2+ , Cu 2+ , and designed a new type of nanocomposite microneedles (ZCQ/MN) containing zinc-copper-quercetin-combined (Zn 2+ , Cu 2+ and Qu) nanospheres, in which the mesoporous nanospheres were functioning as the carrier of metal ions and Qu, and microneedles as the delivery tool for nanospheres

Thiol-containing compounds (RSH) react with DTNB to form mixed disulfides (RS-S-DTNB), where the thiol group replaces one of the nitrobenzoic acid moieties in DTNB