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Table 1 Adrenal hormonal imbalance-associated oxidative stress

Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber

Consistent timing produces consistent tissue concentrations and more reliable results

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