6.3 Toward personalized and mechanism-driven functional eggs Future progress in functional egg development will likely require a transition from population-level fortification to mechanism-driven and personalized nutrition strategies tailored to specific life stages and health conditions, such as aging, pregnancy, and metabolic disorders
Polygenic risk scores (PRS) incorporating PNPLA3, TM6SF2, and MBOAT7 can predict the likelihood of developing liver fat accumulation, inflammation and fibrosis and help to identify individuals at high risk of liver disease progression, particularly in situations where liver biopsy is not possible or practical [281]
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Pregnant or breastfeeding individuals, as well as those with certain medical conditions, should consult with their healthcare provider before considering B12 injections
Cholangiocyte organoid culture For cholangiocyte organoid cultures, EpCAM-positive cholangiocytes were mixed with Matrigel growth factor reduced (Matrigel, Corning) or Cultrex basement membrane extract 2 (BME2) (Cultrex-RGF basement membrane extract type 2, BME2 (AMSBIO) at 50,000 cells per 50 l in each well of a 24-well plate and cultured at 37 C and 5% CO 2 in h-CholOrg EM medium as described in refs
Takimoto, E
