References: Andres, E., Loukili, N
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The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis
Keep enough time for processing, travel, and any clinical questions that may need clarification
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The study sought to clarify whether AOD-9604 produced its reported fat-reducing effects through the same receptor pathway as native growth hormone, and whether the 3-AR pathway was required for activity
