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By monitoring the reduction of phospho-AKT in stimulated RAJI cells (using for example, phospho-AKT antibodies), a compound's potential efficacy in selectively inhibiting PI3K6 can be assessed

For cell treatment by APAP precursors, HepaRG cells and primary human hepatocytes were first incubated with 50 g/ml of APAP or APAP-cys in the maintenance medium described above, during 0.5 h, 2 h, 6 h, 24 h, or 48 h, to optimize the incubation time required to detect APAP metabolites

Regulatory mechanisms controlling gene expression mediated by the antioxidant response element

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Rest assured, severe allergic reactions are highly uncommon, and any discomfort or concerns you might be feeling are likely transitory