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Whilst minor amounts of energy can be produced in the cytoplasm during glycolysis, the major source of cellular energy comes from oxidative phosphorylation, which occurs in the mitochondria

A similar situation was observed in rbohD mutants of Arabidopsis, also exhibiting decreased apoplastic localization (Otulak-Kozie et al., 2023)

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Furthermore, the previous experiments did not rule out the fluorescent labeling of other extracellular material