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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors

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Reconstituted Storage With bacteriostatic water, refrigerate at 28C for up to 4 weeks

doi: 10.1016/j.injury.2004.12.037 182 MorrisGBerkMGaleckiPMaesM

References Frontiers in Endocrinology (2021) Progress on the Function and Application of Thymosin 4 Annals of the New York Academy of Sciences Thymosin 4: roles in development, repair, and engineering of the cardiovascular system Frontiers in Pharmacology (2022) Pharmacokinetics, distribution, metabolism, and excretion of BPC-157 HSS Journal (2025) Emerging Use of BPC-157 in Orthopaedic Sports Medicine: A Systematic Review Current Pharmaceutical Design (2018) Stable Gastric Pentadecapeptide BPC 157: Novel Therapy in Gastrointestinal Tract Journal of Orthopaedic Surgery and Research (2019) BPC 157 and its effects on tendon healing CDC (2024) Vaccine Administration: Subcutaneous (SUBCUT) Injection NCBI Bookshelf (2023) Administration of Parenteral Medications Nursing Skills CDC (NCHS) Laboratory Procedure Manual: C-Peptide in Serum (storage guidelines) Additional Research and Background These sources provide related context and are not presented as support for a specific statement above

Sivaraj, D