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Oxidative stress enhances NF-B-dependent activation of HIV transcription, thereby facilitating viral replication 117

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Jastreboff AM, Kaplan LM, Frias JP, Wu Q, Du Y, Gurbuz S, et al

10.1111/j.1067-1927.2004.12402.x 21 MaquartF

Briefly, LX-2 cells were grown to a confluence of 60-70% on glass coverslips, washed three times with PBS, fixed in 4% formaldehyde for 20 min, permeabilized in 0.5% Triton X-100 for 5 min and blocked with Duolink blocking solution for 60 min at 37 C

However, in allergic patients, particularly during active disease phases, impaired Treg number or function disrupts this regulatory control