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The conjugation reactions transfer functional groups (e.g., glucuronic acid, sulfate, methyl, acetyl) from endogenous molecules to the drug or metabolite, resulting in the formation of highly polar and water-soluble conjugates

Boc-L-Glu-O t Bu was dissolved in THF in the presence of BOP, Castros reagent as the coupling reagent, di-isopropylethylamine (DIEA) as the base, and finally the protected amino acid hydrochloride H-L-Glu (O t Bu)-O t Bu, HCl was added

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Biochemistry 32 , 94869491 (1993)

For instance, in 2022, M