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With regard to gender, there was a significant negative correlation between estrogen level and serum free carnitine in females (58)

The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed

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The cells were then washed with PBS buffer, and 1 10^6 cells were taken for each sample