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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

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BPC-157 has demonstrated potent gastroprotective effects by promoting the healing of damaged GI tissues, reducing inflammation and stomach ulcers, and restoring healthy gut function

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