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Data Processing Single-end reads were generated (i.e., 400 bp/600 bp for fungi and 1,000 bp/1,200 bp for bacteria), followed by qualitative filtering and merging of raw reads by Cutadapt version 1.9.1

The two filler tobaccos were grown as pilot test crops in the 2022/2023 growing season, and because of the limited amount that was grown, there was only enough to make about 3,500 cigars

Scotland introduced the same law in December 2016, and Northern Ireland in February 2022

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