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qRTPCR was performed via SYBR Premix Ex Taq II (Takara) on a Chromo 4 continuous fluorescence detector with a PTC-200 DNA Engine Cycler (Bio-Rad Laboratories, Hercules, CA)

doi:10.1017/s135561770410711x Bienvenu OJ, Friedman LA, Colantuoni E, et al

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For everyone else, the copper delivered by therapeutic doses falls well within safe parameters, with 40+ years of safety data supporting this conclusion