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The next day, cells were washed with PBS and incubated for 3 h in 50 l of choline buffered solution (137.5 mM choline chloride, 5.36 mM KCl, 0.77 mM KH 2 PO 4 , 0.71 mM MgSO 4 .7H 2 O, 1.1 mM CaCl 2 , 10 mM D-glucose and 10 mM HEPES, pH 77.5 at 37 C) containing 600 M L-cystine40 M erastin or 1 mM SAS

2022), indicating that ROS accumulation is likely to be at a lesser extent in cavefish populations, supporting the hypothesis that ROS accumulation creates sleep pressure and ROS is cleared during sleep

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247, Accessed 19 Nov