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doi: 10.1038/s41598-019-39739-5 335 ChengYChenYShangH

OTA affected the gut through decreasing the absorption of nutrients, disrupting the permeability of the intestinal lining, causing cell apoptosis, and modifying the immune system (Liew and Mohd-Redzwan, 2018)

After washing, this was followed by incubation in anti-ATM primary antibody [Y170] (Abcam, Cat# ab32420) diluted 1:100 overnight at 4 C and then incubated in donkey Alexa-488 conjugated secondary antibodies (1:100 dilution, Invitrogen Molecular Probes, UK) for 1 h at RT

That conversation is the responsible first step

E., & Borgwardt, S

The advanced approach ensures the final product delivers the true biological impact demanded by current science