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Otherwise, if the target protein is already purified and should be detected via western blot with Strep-Tactin or Strep-TactinXT conjugates, the application of Biotin Blocking Buffer is possible

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Although the use of targeted metabolite tracing, such as deuterium tracing of NADPH [7], is successful and remains the gold standard in the field, this approach requires mass-spectrometry, which may not be accessible to many laboratories