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Immunoblot A single colony cotransformed with the oxyR-msrA and oxyR-msrB plasmids was inoculated into 10 ml of M9 medium containing 50 g ml 1 kanamycin and 100 g ml 1 ampicillin, followed by overnight incubation at 37 C and 160 rpm

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This hormone has to be at a certain level to be detected by a pregnancy test (3)