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BD 0.9% Sodium Chloride Injection BD Supplied in clear bag for easier visualization of remaining fluids Freeflex bag is a multilayer polyolefin film that is non-PVC and non-DEHP The overwrap is easy to open - simply peel the front and

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injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )

10.1016/j.exer.2015.04.016 [DOI] [PubMed] [Google Scholar] Medvidovic-Grubisic M., Stambolija V., Kolenc D., Katancic J., Murselovic T., Plestina-Borjan I., et al

Rotate between anterior, lateral, and posterior approaches to distribute injection sites

Both camps miss the actual variable: reconstitution sterility and site rotation