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Then, TBST was used to wash the membranes 3 times, followed by incubation with the horseradish peroxidase (HRP)-conjugated secondary antibody (Abcam, United Kingdom) for 1 h at room temperature

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Then cells were washed three times with PBS followed by incubation with the secondary antibody (1:200, Proteintech) and Hoechst 33342 (1:1000) for 1 h

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