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Ethics statement The trial was conducted in accordance with the Declaration of Helsinki (1996 revision) and under the principals of good clinical practice, as laid out in the International Conference on Harmonization document Good Clinical Practice Consolidated Guideline

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Target gene expression levels were normalized by 18 S ribosomal RNA (18 S ribosomal N1), and relative gene expression was calculated using the 2e(-Ct) method 21

Aliquots were centrifuged at 13,000 rpm for 10 min at 4 C, and 150 L supernatant was transferred to Chromacol vials containing 100 L water for dilution

GBD 2019 Diseases and Injuries Collaborators