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Am J Physiol Endocrinol Metab 301:E668E684 Ueki I, Roman HB, Hirschberger LL, Junior CC, Stipanuk MH (2012) Extrahepatic tissues compensate for loss of hepatic taurine synthesis in mice with liver-specific knockout of cysteine dioxygenase

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Western blot The cells were rinsed three times with PBS, and then RIPA buffer (KeyGEN Bio TECH, Nanjing, China) was added to lyse the cells