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By day 2 of the differentiation the cells should have transited through PS to DE, in accordance we observed a down-regulation of MIXL1, GSC, NODAL , and T levels, while markers of DE namely, FOXA2, SOX17, CER1, HHEX and GATA4 were significantly upregulated (see Fig

We tested this hypothesis by injecting Cre-dependent Gsto2 -short hairpin RNA (shRNA) or a scrambled-shRNA viral construct in the striatum of Trkb Penk -KO mice to allow expression of the shRNA specifically in enkephalinergic neurons at 3 months (Fig